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cd138 plasma cell isolation kit  (Miltenyi Biotec)


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    Miltenyi Biotec cd138 plasma cell isolation kit
    Cd138 Plasma Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd138+plasma+cell+isolation+kit/CD138%2B+Plasma+Cell+Isolation+Kit%2C+mouse/us12698321-1147-11-9
    Average 94 stars, based on 20 article reviews
    cd138 plasma cell isolation kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: MZB1 Modulates Inflammatory Severity in Severe Acute Pancreatitis through an IgA-Associated Intestinal Barrier Axis.
    Article Snippet: .. Plasma cells were isolated from wild type mouse spleen using the CD138+ Plasma Cell Isolation Kit (130-092-530, Miltenyi Biotec, Germany) according ..

    Article Title: Discovery and molecular mechanism of potent neutralizing antibody from humanized mice with respiratory syncytial virus.
    Article Snippet: The reaction was terminated by adding an equal volume of ELISA stop solution (Solarbio, C1058), and the absorbance was immediately measured using a multimode plate reader. .. Single B cell screening on Beacon Optofluidic system Plasma B cells were enriched using the CD138+ Plasma Cell Isolation Kit (mouse, Miltenyi Biotec) and the EasySep Mouse CD138 Positive Selection Kit (STEMCELL Technologies). ..

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins.
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19+ B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19+B220+ cells were seeded at a density of 2×106 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×106 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138+ plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Article Title: Antigenic landscape of rabies and related lyssaviruses revealed by cryo-EM
    Article Snippet: Spleens were homogenized via passage three times through a 40μm cell strainer (Corning, #431750) in DMEM with 5% FBS, pooled from both mice, and the resulting single cells were centrifuged at 1400rpm for 8 min at 4°C. .. Bulk splenocytes were subject to a non-plasma cell depletion cocktail using the CD138 + plasma cell isolation kit, (Miltenyi Biotec, #130-092-530) according to manufacturer’s instructions. .. Plasma cells were then purified using the EasySep Release Mouse CD138+ kit (STEMCELL Technologies, #100-0601) according to the manufacturer’s instructions.

    Article Title: Antibodies which have specificity to a sarbecovirus spike protein receptor-binding domain and methods of use thereof for treating a coronavirus infection
    Article Snippet: .. Plasma B cells were isolated by CD138+ cell enrichment (Miltenyi Biotec CD138+ plasma cell isolation kit, catalog no. 130-092-530). .. Enriched plasma B cell samples were loaded onto an OptoSelect 11k chip (Berkeley Lights, Inc., Emeryville, CA) in BLI Mouse Plasma Cell Media (Berkeley Lights, Inc., catalog no. 750-70004).

    Article Title: Broad and durable protection against SARS-CoV-2 and SARS-CoV by an intranasal chimpanzee adenovirus vaccine expressing tandem RBDs and nucleocapsid
    Article Snippet: Polystyrene particles (Spherotech, USA) were coated with RBD proteins (0.5 mg/mL) for 1 h at 4 °C and washed three times with PBS. .. Bone marrow ASCs were enriched using the CD138 + Plasma Cell Isolation Kit (Miltenyi Biotec, Cat. #130-092-530) and incubated for 1 h at 37 °C with RBD–coated polystyrene particles, AF561-labeled anti-mouse IgG (Thermo Fisher, USA), and APC-labeled anti-mouse IgA (Southern Biotech, Cat. #1165-11) on the MoSMAR-chip platform. .. RBD-specific ASCs were identified by fluorescence microscopy (Olympus, Japan), isolated with a microneedle, and transferred into lysis buffer in microwells.

    Article Title: Cinobufagin overcomes bortezomib resistance in multiple myeloma strains by targeting SEC62/TRPM4-mediated NECSO
    Article Snippet: Background: Necrosis by sodium overload (NECSO) is a poorly understood, novel form of cell death implicated in cancer.. TRPM4 is the only protein currently linked to NECSO and is downregulated in bortezomib-resistant multiple myeloma (MM) patients and cell lines.. Purpose: To investigate the potential of cinobufagin to overcome bortezomib resistance in MM and elucidate its underlying mechanism of action, particularly regarding TRPM4 and NECSO induction.

    Article Title: Discovery and molecular mechanism of potent neutralizing antibody from humanized mice with respiratory syncytial virus
    Article Snippet: The reaction was terminated by adding an equal volume of ELISA stop solution (Solarbio, C1058), and the absorbance was immediately measured using a multimode plate reader. .. Plasma B cells were enriched using the CD138 + Plasma Cell Isolation Kit (mouse, Miltenyi Biotec) and the EasySep Mouse CD138 Positive Selection Kit (STEMCELL Technologies). ..

    Isolation:

    Article Title: MZB1 Modulates Inflammatory Severity in Severe Acute Pancreatitis through an IgA-Associated Intestinal Barrier Axis.
    Article Snippet: .. Plasma cells were isolated from wild type mouse spleen using the CD138+ Plasma Cell Isolation Kit (130-092-530, Miltenyi Biotec, Germany) according ..

    Article Title: Antibodies which have specificity to a sarbecovirus spike protein receptor-binding domain and methods of use thereof for treating a coronavirus infection
    Article Snippet: .. Plasma B cells were isolated by CD138+ cell enrichment (Miltenyi Biotec CD138+ plasma cell isolation kit, catalog no. 130-092-530). .. Enriched plasma B cell samples were loaded onto an OptoSelect 11k chip (Berkeley Lights, Inc., Emeryville, CA) in BLI Mouse Plasma Cell Media (Berkeley Lights, Inc., catalog no. 750-70004).

    Cell Isolation:

    Article Title: MZB1 Modulates Inflammatory Severity in Severe Acute Pancreatitis through an IgA-Associated Intestinal Barrier Axis.
    Article Snippet: .. Plasma cells were isolated from wild type mouse spleen using the CD138+ Plasma Cell Isolation Kit (130-092-530, Miltenyi Biotec, Germany) according ..

    Article Title: Discovery and molecular mechanism of potent neutralizing antibody from humanized mice with respiratory syncytial virus.
    Article Snippet: The reaction was terminated by adding an equal volume of ELISA stop solution (Solarbio, C1058), and the absorbance was immediately measured using a multimode plate reader. .. Single B cell screening on Beacon Optofluidic system Plasma B cells were enriched using the CD138+ Plasma Cell Isolation Kit (mouse, Miltenyi Biotec) and the EasySep Mouse CD138 Positive Selection Kit (STEMCELL Technologies). ..

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins.
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19+ B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19+B220+ cells were seeded at a density of 2×106 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×106 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138+ plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Article Title: Antigenic landscape of rabies and related lyssaviruses revealed by cryo-EM
    Article Snippet: Spleens were homogenized via passage three times through a 40μm cell strainer (Corning, #431750) in DMEM with 5% FBS, pooled from both mice, and the resulting single cells were centrifuged at 1400rpm for 8 min at 4°C. .. Bulk splenocytes were subject to a non-plasma cell depletion cocktail using the CD138 + plasma cell isolation kit, (Miltenyi Biotec, #130-092-530) according to manufacturer’s instructions. .. Plasma cells were then purified using the EasySep Release Mouse CD138+ kit (STEMCELL Technologies, #100-0601) according to the manufacturer’s instructions.

    Article Title: Antibodies which have specificity to a sarbecovirus spike protein receptor-binding domain and methods of use thereof for treating a coronavirus infection
    Article Snippet: .. Plasma B cells were isolated by CD138+ cell enrichment (Miltenyi Biotec CD138+ plasma cell isolation kit, catalog no. 130-092-530). .. Enriched plasma B cell samples were loaded onto an OptoSelect 11k chip (Berkeley Lights, Inc., Emeryville, CA) in BLI Mouse Plasma Cell Media (Berkeley Lights, Inc., catalog no. 750-70004).

    Article Title: Broad and durable protection against SARS-CoV-2 and SARS-CoV by an intranasal chimpanzee adenovirus vaccine expressing tandem RBDs and nucleocapsid
    Article Snippet: Polystyrene particles (Spherotech, USA) were coated with RBD proteins (0.5 mg/mL) for 1 h at 4 °C and washed three times with PBS. .. Bone marrow ASCs were enriched using the CD138 + Plasma Cell Isolation Kit (Miltenyi Biotec, Cat. #130-092-530) and incubated for 1 h at 37 °C with RBD–coated polystyrene particles, AF561-labeled anti-mouse IgG (Thermo Fisher, USA), and APC-labeled anti-mouse IgA (Southern Biotech, Cat. #1165-11) on the MoSMAR-chip platform. .. RBD-specific ASCs were identified by fluorescence microscopy (Olympus, Japan), isolated with a microneedle, and transferred into lysis buffer in microwells.

    Article Title: Cinobufagin overcomes bortezomib resistance in multiple myeloma strains by targeting SEC62/TRPM4-mediated NECSO
    Article Snippet: Background: Necrosis by sodium overload (NECSO) is a poorly understood, novel form of cell death implicated in cancer.. TRPM4 is the only protein currently linked to NECSO and is downregulated in bortezomib-resistant multiple myeloma (MM) patients and cell lines.. Purpose: To investigate the potential of cinobufagin to overcome bortezomib resistance in MM and elucidate its underlying mechanism of action, particularly regarding TRPM4 and NECSO induction.

    Article Title: Discovery and molecular mechanism of potent neutralizing antibody from humanized mice with respiratory syncytial virus
    Article Snippet: The reaction was terminated by adding an equal volume of ELISA stop solution (Solarbio, C1058), and the absorbance was immediately measured using a multimode plate reader. .. Plasma B cells were enriched using the CD138 + Plasma Cell Isolation Kit (mouse, Miltenyi Biotec) and the EasySep Mouse CD138 Positive Selection Kit (STEMCELL Technologies). ..

    Selection:

    Article Title: Discovery and molecular mechanism of potent neutralizing antibody from humanized mice with respiratory syncytial virus.
    Article Snippet: The reaction was terminated by adding an equal volume of ELISA stop solution (Solarbio, C1058), and the absorbance was immediately measured using a multimode plate reader. .. Single B cell screening on Beacon Optofluidic system Plasma B cells were enriched using the CD138+ Plasma Cell Isolation Kit (mouse, Miltenyi Biotec) and the EasySep Mouse CD138 Positive Selection Kit (STEMCELL Technologies). ..

    Article Title: Discovery and molecular mechanism of potent neutralizing antibody from humanized mice with respiratory syncytial virus
    Article Snippet: The reaction was terminated by adding an equal volume of ELISA stop solution (Solarbio, C1058), and the absorbance was immediately measured using a multimode plate reader. .. Plasma B cells were enriched using the CD138 + Plasma Cell Isolation Kit (mouse, Miltenyi Biotec) and the EasySep Mouse CD138 Positive Selection Kit (STEMCELL Technologies). ..

    Concentration Assay:

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins.
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19+ B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19+B220+ cells were seeded at a density of 2×106 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×106 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138+ plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    FACS:

    Article Title: Interleukin 35-producing B cells prolong the survival of GVHD mice by secreting exosomes with membrane-bound IL-35 and upregulating PD-1/LAG-3 checkpoint proteins.
    Article Snippet: For producing i35 Bregs used for treatment of GVHD mice, CD19+ B cells were isolated from the spleen of donor B6C3F1/Cr1 mice using CD19 specific magnetic microbeads (Miltenyi Biotec, San Diego, CA). .. The CD19+B220+ cells were seeded at a density of 2×106 cells/mL, stimulated with LPS (5 μg/mL) or anti-IgM (5 μg/mL) with anti-CD40 (5 μg/mL) and rIL-35 (50 ng/mL) at a concentration of 2×106 cells/mL for 72 h. The cells were enriched for CD138-expressing B cells using CD138 antibody and sorted on FACS Aria or with Mouse CD138 microbeads from CD138+ plasma cell isolation kit (Miltenyi Biotec, San Diego, CA). ..

    Incubation:

    Article Title: Broad and durable protection against SARS-CoV-2 and SARS-CoV by an intranasal chimpanzee adenovirus vaccine expressing tandem RBDs and nucleocapsid
    Article Snippet: Polystyrene particles (Spherotech, USA) were coated with RBD proteins (0.5 mg/mL) for 1 h at 4 °C and washed three times with PBS. .. Bone marrow ASCs were enriched using the CD138 + Plasma Cell Isolation Kit (Miltenyi Biotec, Cat. #130-092-530) and incubated for 1 h at 37 °C with RBD–coated polystyrene particles, AF561-labeled anti-mouse IgG (Thermo Fisher, USA), and APC-labeled anti-mouse IgA (Southern Biotech, Cat. #1165-11) on the MoSMAR-chip platform. .. RBD-specific ASCs were identified by fluorescence microscopy (Olympus, Japan), isolated with a microneedle, and transferred into lysis buffer in microwells.



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